ANTIBACTERIAL ACTIVITY OF HONEY ON Staphylococcus aureusEscherichia coli and Streptococcus pyogen - Easy Project Materials Website, Free Research Project Topics And Materials

ANTIBACTERIAL ACTIVITY OF HONEY ON Staphylococcus aureusEscherichia coli and Streptococcus pyogen

ISOLATED FROM WOUND

Click here to download our android mobile app to your phone for more materials and others

CHAPTER ONE
1.0	Introduction	1
1.1	Aims and objectives	3
CHAPTER TWO
2.0	Literature review	5

2.1	Wound infection		8
2.2 Definition of honey			9
2.3		Local test for honey	13
2.4		Classification of honey	14
2.5		Preservation of honey	16
2.6		Properties and active ingredient of honey	17
2.7		Mode of action of some antibacterial substance
		present in honey	20
2.8		Clinical conditions for treatment with honey	22
2.9		Honey as an antimicrobial agent	24
2.10		Practical consideration for the clinical use of honey	27
2.11		Adverse reaction of honey	28
2.12 Research on honey			29

CHAPTER THREE
3.1	Source of Sample	32
3.2	Sources of honey	32
3.3	Identification of organisms	32
3.4	Gram Staining	33
3.5	Indole test	34
3.6	Catalase test	34
3.7	Coagulase test	35
3.8	Antibacterial sensitivity test	35
	CHAPTER FOUR
4.1	Result	37

vii

CHAPTER FIVE
5.1	Discussion	40
5.2	Conclusion	42
5.3	Recommendation	43
REFERENCE		44
Appendix 1		51
Appendix 2		54

viii

	LIST OF TABLES
Table 1	– Composition of honey	13
Table 2	– Result of Biochemical tests	25
Table 3	– Inhibition of honey from Enugu North (Nsukka)	26
Table 4	– Inhibition of honey from Enugu South (Ugwuaji)	26

For more topics click

ABSTRACT

Antibacterial activity of honey obtained from two different locations in Delta State (Abraka & Obiaru) Nigeria on Staphylococcus aureus, Escherichia coli and Streptococcus pyogens isolated from wound was studied. Agar well diffusion method was used to determine the antibacterial activity of the honey on the test microorganisms. The result revealed that the two honey samples have heavy antibacterial activities against the test organisms and zones of inhibition were obtained showing high antibacterial activity. The antibacterial activity increased with increase in the concentrations and honey from Abraka produced a high antibacterial activity (clearer zone) on staphylococcus aureus and Escherichia coli at all concentration and moderately for streptococcus pyogens. The use of honey as a therapeutic substance has been rediscovered by the medical profession on more recent times, and it is gaining acceptance as an antibacterial agent for the treatment of ulcers and bed sores, and other infections resulting from burns and wounds.

CHAPTER ONE

1.0 Introduction

Infections and other health related problems have been of great concern to human beings and chemotherapy is the main approach in the treatment of such conditions. Investigation into the microbial flora of wound began in the late 19^th century and since then; improvements in techniques have facilitated the recovery, identification and enumeration of a wide variety of microbial species. Most wounds support relatively stable polymicrobial communities (Bowkler, et.al; 2001) often without signs of clinical infection (Hansson,et al; 1993).

However, potential pathogens may be present and the delicate balance between colonized wound and an infected wound depends on the interplay of complex host and microbial influences (Emmerson, 1998). The development of wound infection has deleterious effect on

patients by causing increased pain, discomfort, inconveniences and can lead to life threatening conditions or even death.

Major challenges encountered with antibiotics in clinical use are resistance to antibiotics which leads eventually to failure of the treatment (Blair 2004). Infectious diseases are known to be treated with herbal remedies throughout the history of mankind; even today, natural substances continue to play a major role in primary health care as therapeutic remedies in many developing countries (Jonathan, et.al; 2007). Over the years, there have been reports of the production of more potent antibiotics e.g. third and fourth generation of cephalosporin by pharmaceutical companies which are not readily available and expensive. Problems of various antibiotics include low efficacy, side effect which has lead investigations into natural and potent antibacterial seeming to be the right step to take. The invasion of pathogenic organism is on the rise as a result, effects are been made to develop antibacterial agent from natural sources for better

therapeutic effect (Gills, 1992). The therapies have drawn the interest of both public and medicinal communities. Current research has been focused on herbal and aromatherapy product. However, a number of their product such as honey has shown therapeutic promise.

The presence in honey of various inhibins as described by (Doid and Dzaio, 1937) has been reported by several investigators. Honey was used to treat infected wound as long as 2000 years ago before bacterial were discovered to be the cause of infection in c.50 AD,

Dioscorides described honeyandhollow as be ulcers” 1959)(Gunther,.Morerecently,honey has been reported to

have an inhibitory effect to around 60 species of bacterial including aerobes and anaerobes, Gram positive and Gram negative (Molan, 1992). The current prevalence of the therapeutic use of ancient remedies, include honey committee on science and technology.

The relevance of population census to economic development of rural areas in nigeria

One thought on “ANTIBACTERIAL ACTIVITY OF HONEY ON Staphylococcus aureusEscherichia coli and Streptococcus pyogen”

Leave a Reply Cancel reply

all project topics and materials ,ASSISTING STUDENTS IN ACHIEVING EXCELLENT PROJECT WORK