Comparative Analysis Of Antimicrobial Strength Of Three Most Common Antibiotics Used In Enugu

(Case Study Of Antibiotics Drugs Brought In Obiagu.)

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ABSTRACT

In comparing the antimicrobial strength of three most common antibiotics, which includes: streptomycin chloranphenicol and gentarccin.
Samples of urine and high vaginal swab (H.V.S) were collected from park lane Enugu. The organisms isolated were pure culture of staphylococcus aureus and Escherichia coli
Sensitivity test was carried out with the use of sensitivity disk containing various minimum inhibitory concentration of the different antibiotics.
The result obtained shaved that gentamicn was more effective followed by chloramphenicol while some organisms shaved resistant to streptomycin.

LIST OF TABLE

TABLE 1 NUTRIENT AGAR
TABLE 2 BLOOD AGAR
TABLE 3: ANTIBIOTICS AGAINST SYAPHYLOCOCCUS AUREUS
TABLE 4: ANTIBIOTIC AGAINST ESCHERICHIA COLI
LIST OF FIGURES

FIGURE 1: STREPTOMYCIN
FIGURE 2: CHLORAMPHENICOL
FIGURE3: SENSITIVITY TEST ON STAPHYLOCOCCUS AUREUS
FIGURE 4 SENSITIVITY TEST ON ESCHERICHIA COLI
FIGURE 5: STAPHYLOCOCCUS AUREUS AGAINST ANTIBIOTICS
FIGURE 6: ESCHERICHIA COLI AGAINST ANTIBIOTICS
TABLE OF CONTENT

CHAPTER ONE
1.0 Introduction
1.1 Historical background
1.2 Aims and objective
1.3 Hypothesis
1.4 Statement of problem
1.5 Scope of study
1.6 Limitation of study

CHAPTER TWO
2.0 Literature review
3.1 Procurement of antibiotics
3.1.1 streptomycin
2.1.2 Chloramphenicol
2.1.3 Gentamicin
2.2 Antimicrobial activity in vitro
2.3 Antimicrobial activity in vivo
2.4 Resistance to antimicrobial drugs
2.5 Factors affecting antimicrobial activity
2.6 Clinical use of antibiotics
2.7 Characteristic of antibiotics

CHAPTER THREE
3.0 Materials and method
3.1 Sample collection
3.1.1 Media composition
3.1.2 Direction for the preparation of nutrient agar
3.1.3 Direction for the preparation of blood agar
3.1.4 Material used for the prewritten of media

CHAPTER FOUR
4.0 Isolation and identification procedures
4.1.1 Isolation and identification of staphylococcus aureus
4.1.2 Isolation and identification of escherichia coli
4.2 Sensitivity test

CHAPTER FIVE
5.0 RECOMMENDATION
5.1 CONCLUSION
REFERENCES

CHAPTER ONE

INTRODUCTION
1.1 HISTORICAL BACK GROUND
Antibiotics are chemicals when the chemical are put into the body, they stop the growth of kinds of germs. They help the body to fight diseases. More than 3,000 years ago ancient people stumbled over the discovery that some moulds could be used as a cure. The egyptians, the chinese, and indians of central American would use mold to treat rashes and infected would. At that time they didn’t understand either diseases or treatment. As time went on, people began to gain some insight of disease. In the 1860 Louis Pasteur Shaw that many disease were caused it bacteria. Later he discovered that we may be able to fight germ and other microbes. It was two German doctors, who were first to make an effective medication form microbes. Kudo if and Emmerich and Oscar has conducted their experiment in the 1890. They proved that germ that would for another. All the men did was to take the germ from infected bandages and grow then in a test tube. They would then isolate a particular germ that caused green in factions in open would. This germs was bacteria called Bacillus pyoicyaneus. They put then into another test tube containing other type of bacteria, it was then it happened that the bacillus pyocyaneus wiped out the other disease germ. The germs that was killed were those that caused cholera, typhoid, diphtheria and anthrau. From this the two men created a medication that they called pyoanase, it was the first antibiotics used in hospitals.

In 1928 Alexander Fleming, a Scottish scientist, discovered penicillin, the first antibiotics. He was keeping in a petn dish when a speck of mold fell in, it cause the mold to grow on the nutrient agar used to feed the bacteria. Surpassingly, it stopped the growth of the bacteria. Fleming through the mold called penicillin notatum produced a substance that killed the bacteria and so called it penicillin. However, he was not able to entrant it from..

Comparative Analysis Of Antimicrobial Strength Of Three Most Common Antibiotics Used In Enugu

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